is there is only a TIFR research centre in pune, is there are other institute? please tell the qualification needed for appearing for Phd

You have not given your education background and the field of Ph.D. Yet, I will try to answer you keeping in mind that you are science scholar. In Pune, Symbiosis, BVU college of engineering, University of Pune, MIT etc offer Ph.D program. The minimum qualification is masters degree with 60% marks