Which one of the following techniques is not used to spot components of a mixture separated in thin layer chromatographic plate?
Which one of the following techniques is not used to spot components of a mixture separated in thin layer chromatographic plate?
Option 1 -
I2 (Solid)
Option 2 -
U.V Light
Option 3 -
Visualisation agent as a component of mobile phase
Option 4 -
Spraying of an appropriate reagent
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1 Answer
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Correct Option - 2
Detailed Solution:In thin layer chromatography, the spots of colourless compounds, which are invisible to eyes can be detected by putting the plate under UV light.
Another detection technique is to place the plate in a covered jar containing I2 (s). Sometimes an appropriate reagent may also be sprayed on the plate, ninhydrin in case of amino acids.
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